GUIDELINES FOR EM FINAL REPORT

Your final report must be typed and presented in the format of a scientific paper. It should be checked for spelling and grammar. You will need to have at least 2 primary references that you cite to explain the science of your paper. A primary reference is a scientific paper that reports results of original research. Review articles, text books and Internet sites are not primary references. You may need, however, to cite these or additional papers in reference to the techniques you use. The paper should have the following sections:

Please note that an abstract is not necessary

Introduction - what was the background and hypothesis for your study?

Materials and Methods - what did you do to treat and prepare your tissue. You do not need to tell all the times (in alcohols for example) if they represent a standard protocol.

Results - this section will include a textual description of your results and the pictures with figure legends. Please note that merely giving photos with figure legends is insufficient. Consult me or an EM paper if you have questions about this. Your photos should be included in this section, and should be annotated with labels. The magnification of the photo should be given. Please consult the syllabus for the number of photos required. Fewer photos will result in a reduction in points. The quality of the micrographs will be assessed as an indication of your proficiency. More practice will give you more proficiency - there is plenty of lab time to develop the proficiency required.

Discussion - in this section you will tell how what you saw either agrees with or augments currently published studies. If there are no EM studies of your tissue, how does what you found relate to physiology of the organism, or to EM or physiology of similar organisms?

Literature cited - Citations of the primary and secondary references cited in your paper. You should use the following format for citations – deviations from this will affect your grade:

Within the body of the paper:

….Plants are known to have 2 methods of coping with aluminum stress (Jones and McCoy, 1997; Smith et al., 1998).

In the Literature Cited section the papers would be referenced as follows:

Jones, D.B. and M.N Smith. 1997. Responses of Glycine max seedlings to three levels of aluminum ion. Physiol. Plant. 45: 677-698.

Smith, M.N., L.G. Rach and D.B. Jones. 1998. Growth of corn plants under high levels of aluminum and varying pH. New Phytol. 33:990-996.

For an article from a book the citation would be;

Jones, D.B. and M.N. Smith. 1997. Responses of Glycine max seedlings to three levels of aluminum ion. In: D.R. Konally (ed.) Plants and Ion Metabolism. Wiley Publishers. pp. 477-498.

If you have any questions, please ask.