WWWtacg 3 Analysis of L4440gtwy predicted sequence È For pasted-in sequence, labelled L4440gtwy == Sequence info: 4387 bases; 1135 A(25.87 %) 1020 C(23.25 %) 1065 G(24.28 %) 1167 T(26.60 %) == Enzymes that DO NOT MAP to this sequence: AgeI StuI XbaI == Total Number of Hits per Enzyme: ApaI 1 EcoRI 1 MluI 1 BamHI 2 HincII 3 StyI 1 DraI 5 HindIII 1 == Match Sites by Enzyme or Pattern *Maximum* Hits: 50 ApaI G_GGCC'C (0 Err) - 1 Match(s) found (1.58 sites exp) 1805 EcoRI G'AATT_C (0 Err) - 1 Match(s) found (2.35 sites exp) 506 HindIII A'AGCT_T (0 Err) - 1 Match(s) found (2.35 sites exp) 1771 MluI A'CGCG_T (0 Err) - 1 Match(s) found (1.92 sites exp) 953 StyI C'CwwG_G (0 Err) - 1 Match(s) found (7.70 sites exp) 807 BamHI G'GATC_C (0 Err) - 2 Match(s) found (1.92 sites exp) 256 959 HincII GTy'rAC (0 Err) - 3 Match(s) found (8.52 sites exp) 1098 1636 1788 DraI TTT'AAA (0 Err) - 5 Match(s) found (2.86 sites exp) 426 765 2802 3494 3513 == Pseudo-Gel Map of Digestions: *Maximum* Hits: 50 # frags 10 100 1000 10000 . . . . . ..... . . . . ..... . . . . ..... ApaI | 1 EcoRI | 1 HindIII | 1 MluI | 1 . . . . . ..... . . . . ..... . . . . ..... StyI | 1 BamHI | | 2 HincII | | | 3 DraI | | | | | 5 == Fragment Sizes by Enzyme or Pattern (SORTED) *Maximum* Hits: 50 BamHI G'GATC_C (0 Err) - 2 Fragment(s) found (1.92 sites exp) 703 3684 HincII GTy'rAC (0 Err) - 3 Fragment(s) found (8.52 sites exp) 152 538 3697 DraI TTT'AAA (0 Err) - 5 Fragment(s) found (2.86 sites exp) 19 339 692 1300 2037 Analyze just the GTWY cassette (as linear) == Sequence info: 1717 bases; 484 A(28.19 %) 349 C(20.33 %) 404 G(23.53 %) 480 T(27.96 %) == Enzymes that DO NOT MAP to this sequence: AgeI ApaI HindIII StuI XbaI == Total Number of Hits per Enzyme: BamHI 2 EcoRI 1 MluI 1 DraI 2 HincII 2 StyI 1 == Match Sites by Enzyme or Pattern *Maximum* Hits: 50 EcoRI G'AATT_C (0 Err) - 1 Match(s) found (1.02 sites exp) 454 MluI A'CGCG_T (0 Err) - 1 Match(s) found (0.62 sites exp) 901 StyI C'CwwG_G (0 Err) - 1 Match(s) found (2.48 sites exp) 755 BamHI G'GATC_C (0 Err) - 2 Match(s) found (0.62 sites exp) 204 907 DraI TTT'AAA (0 Err) - 2 Match(s) found (1.68 sites exp) 374 713 HincII GTy'rAC (0 Err) - 2 Match(s) found (3.23 sites exp) 1046 1584 == Pseudo-Gel Map of Digestions: *Maximum* Hits: 50 # frags 10 100 1000 10000 . . . . . ..... . . . . ..... . . . . ..... EcoRI | | 2 MluI || 2 StyI | | 2 BamHI | || 3 . . . . . ..... . . . . ..... . . . . ..... DraI 2 | 3 HincII | | | 3 == Fragment Sizes by Enzyme or Pattern (SORTED) *Maximum* Hits: 50 EcoRI G'AATT_C (0 Err) - 2 Fragment(s) found (1.02 sites exp) 454 1263 MluI A'CGCG_T (0 Err) - 2 Fragment(s) found (0.62 sites exp) 816 901 StyI C'CwwG_G (0 Err) - 2 Fragment(s) found (2.48 sites exp) 755 962 BamHI G'GATC_C (0 Err) - 3 Fragment(s) found (0.62 sites exp) 204 703 810 DraI TTT'AAA (0 Err) - 3 Fragment(s) found (1.68 sites exp) 339 374 1004 HincII GTy'rAC (0 Err) - 3 Fragment(s) found (3.23 sites exp) 133 538 1046 EcoRI / HindIII double digest frags: 1265, 3122 More enzymes: BglII A'GATC_T (0 Err) - 1 Match(s) found 48 KpnI G_GTAC'C (0 Err) - 1 Match(s) found (1.92 sites exp) 1811 PstI C_TGCA'G (0 Err) - 1 Match(s) found (1.92 sites exp) 1632 XhoI C'TCGA_G (0 Err) - 1 Match(s) found (1.92 sites exp) 1792 XmaI C'CCGG_G (0 Err) - 1 Match(s) found (1.58 sites exp) 1375