Hobert lab wheel of PCR troubleshooting


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PCR troubleshooter choices

PCR troubleshooter
What was wrong?
  1. Forgot to add primers
  2. Forgot to add Taq
  3. T annealing too high!
  4. That PCR machine is broken
  5. Time of extension too short!
  6. Did you add dNTPs??
  7. Forgot to add DNA template
  8. Nothing wrong. Just repeat the PCR, eat a chocholate and relax!!