Sequence from M13 forward (-20) primer to M13 reverse primer, divided on lines as in the image above. [Note that we are typically sequencing now using the GW1 or GW2 primer, closer to the insert.
TGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATT TTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAA AAAGCAGGCTCCGAATTCGCCCTT-"PCR_product_insert"-AAGGGCGAATTCGACCCAGCTTTCTTGTACAAA GTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATT ATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGReverse complement of this sequence (same line arrangement):
CCAGGAAACAGCTATGACCATGTAATACGACTCACTATAGGGGATATCAGCTGGATGGCAAAT AATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATTATTTTTTATAATGCCAAC TTTGTACAAGAAAGCTGGGTCGAATTCGCCCTT-"PCR_product_insert"AAGGGCGAATTCGGAGCCTGCTTT TTTGTACAAAGTTGGCATTATAAAAAAGCATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAA AATCATTATTTGGGGCCCGAGCTTAAGACTGGCCGTCGTTTTACA