Biology 376 Links for unc-86 - POU domain proteins lab
C. elegans unc-86 + POU-domain proteins + terminal selector lab GROUP TASKS
Most can be done in any order - so while one group is using the microscope, others can do some of the computer-based tasks
- Prepare a multiple sequence alignment of C. elegans unc-86 (top) with other nematode unc-86 orthologs (or at least POU domain proteins), plus human Brn3b and POU4 class proteins.
- Photograph a z-series of at least one high quality stained adult or larva of your species of interest (either Oscheius tipulae [strain CEW1] or Pangrolaimus sp. [strain PS1159]. (Dr. Loer will assist in getting you started, and selecting a high quality specimen from the slide.)
- Transfer your images to a separate computer. Download FIJI and use it to convert your images into a stack [Images: Stack: Images to Stack]. Play with brightness & contrast to optimize image.
- Determine the pattern of expression (make a schematic image) for your species of one larva and one adult. (There are some already prepared stacks online.) Compare to the C. elegans pattern of expression (see schematics in the resources folder), and speculate on how they are different - can you identify any likely 'homologous' cells in the other species?
- For next Wednesday (Dec 11), prepare a brief presentation with your group with an introduction to terminal selectors, your nematode of interest, unc-86, and then show your data and results - alignment(s) and pattern of expression in an adult and larva. (Inculding compare/contrast with C. elegans)
C. elegans unc-86 + POU-domain proteins + terminal selector papers
See the Literature folder
Terminal selectors / Neuronal identity genes
Deneris&Hobert2014_Maintenance_of_neuronal_identity_rev
Hobert2016_Ce_terminal selectors_of_neuronal_identity_review
Hobert2016_Map_of_Ce_terminal_regulators
POU domain TFs / C.e. unc-86 / Mammalian Brn3
Baumeister1996_Ce_unc-86_lineage_asymmetry
Finney1986_Ce_unc-86_encodes_homeodomain_TF
Finney&Ruvkun1990_Ce_unc-86_cell_identity_&_lineage
Flames2011_Monoamine_fate_review
Gold2014_Evolution_of_POU_domain_proteins
Herr1988_POU_domain_description
Sze1997_Ce_unc-86_VP16-activation_causes_defects
Sze2002_Ce_unc-86_regulates_tph-1_expression
Sze2003_Ce_unc-86_regulates_olfactory_sensitivity
Zhang2014_Ce_ttx-3_unc-86_serotonergic_identity
In WormBook:
Nematodes other than C. elegans
Oscheius tipulae
Links for C. elegans unc-86 + POU-domain proteins + terminal selector lab
POU domain protein sequence motif
NCBI Blast page
Instructions for doing multiple sequence alignments (MSAs)
Clustal Omega for MSAs
WormBase
Pristionchus-specific Blast page
Links for unc-86-related images and image analysis
FIJI downloads
Folder with unc-86 cell schematics from C. elegans, plus other resources
Folder with unc-86 antibody stained worms