WWWtacg 3 Analysis of L4440gtwy predicted sequence
È For pasted-in sequence, labelled L4440gtwy
== Sequence info:
4387 bases; 1135 A(25.87 %) 1020 C(23.25 %) 1065 G(24.28 %) 1167 T(26.60 %)
== Enzymes that DO NOT MAP to this sequence:
AgeI StuI XbaI
== Total Number of Hits per Enzyme:
ApaI 1 EcoRI 1 MluI 1
BamHI 2 HincII 3 StyI 1
DraI 5 HindIII 1
== Match Sites by Enzyme or Pattern
*Maximum* Hits: 50
ApaI G_GGCC'C (0 Err) - 1 Match(s) found (1.58 sites exp)
1805
EcoRI G'AATT_C (0 Err) - 1 Match(s) found (2.35 sites exp)
506
HindIII A'AGCT_T (0 Err) - 1 Match(s) found (2.35 sites exp)
1771
MluI A'CGCG_T (0 Err) - 1 Match(s) found (1.92 sites exp)
953
StyI C'CwwG_G (0 Err) - 1 Match(s) found (7.70 sites exp)
807
BamHI G'GATC_C (0 Err) - 2 Match(s) found (1.92 sites exp)
256 959
HincII GTy'rAC (0 Err) - 3 Match(s) found (8.52 sites exp)
1098 1636 1788
DraI TTT'AAA (0 Err) - 5 Match(s) found (2.86 sites exp)
426 765 2802 3494 3513
== Pseudo-Gel Map of Digestions:
*Maximum* Hits: 50
# frags
10 100 1000 10000
. . . . . ..... . . . . ..... . . . . .....
ApaI | 1
EcoRI | 1
HindIII | 1
MluI | 1
. . . . . ..... . . . . ..... . . . . .....
StyI | 1
BamHI | | 2
HincII | | | 3
DraI | | | | | 5
== Fragment Sizes by Enzyme or Pattern (SORTED)
*Maximum* Hits: 50
BamHI G'GATC_C (0 Err) - 2 Fragment(s) found (1.92 sites exp)
703 3684
HincII GTy'rAC (0 Err) - 3 Fragment(s) found (8.52 sites exp)
152 538 3697
DraI TTT'AAA (0 Err) - 5 Fragment(s) found (2.86 sites exp)
19 339 692 1300 2037
Analyze just the GTWY cassette (as linear)
== Sequence info:
1717 bases; 484 A(28.19 %) 349 C(20.33 %) 404 G(23.53 %) 480 T(27.96 %)
== Enzymes that DO NOT MAP to this sequence:
AgeI ApaI HindIII StuI XbaI
== Total Number of Hits per Enzyme:
BamHI 2 EcoRI 1 MluI 1
DraI 2 HincII 2 StyI 1
== Match Sites by Enzyme or Pattern
*Maximum* Hits: 50
EcoRI G'AATT_C (0 Err) - 1 Match(s) found (1.02 sites exp)
454
MluI A'CGCG_T (0 Err) - 1 Match(s) found (0.62 sites exp)
901
StyI C'CwwG_G (0 Err) - 1 Match(s) found (2.48 sites exp)
755
BamHI G'GATC_C (0 Err) - 2 Match(s) found (0.62 sites exp)
204 907
DraI TTT'AAA (0 Err) - 2 Match(s) found (1.68 sites exp)
374 713
HincII GTy'rAC (0 Err) - 2 Match(s) found (3.23 sites exp)
1046 1584
== Pseudo-Gel Map of Digestions:
*Maximum* Hits: 50
# frags
10 100 1000 10000
. . . . . ..... . . . . ..... . . . . .....
EcoRI | | 2
MluI || 2
StyI | | 2
BamHI | || 3
. . . . . ..... . . . . ..... . . . . .....
DraI 2 | 3
HincII | | | 3
== Fragment Sizes by Enzyme or Pattern (SORTED)
*Maximum* Hits: 50
EcoRI G'AATT_C (0 Err) - 2 Fragment(s) found (1.02 sites exp)
454 1263
MluI A'CGCG_T (0 Err) - 2 Fragment(s) found (0.62 sites exp)
816 901
StyI C'CwwG_G (0 Err) - 2 Fragment(s) found (2.48 sites exp)
755 962
BamHI G'GATC_C (0 Err) - 3 Fragment(s) found (0.62 sites exp)
204 703 810
DraI TTT'AAA (0 Err) - 3 Fragment(s) found (1.68 sites exp)
339 374 1004
HincII GTy'rAC (0 Err) - 3 Fragment(s) found (3.23 sites exp)
133 538 1046
EcoRI / HindIII double digest frags: 1265, 3122
More enzymes:
BglII A'GATC_T (0 Err) - 1 Match(s) found 48
KpnI G_GTAC'C (0 Err) - 1 Match(s) found (1.92 sites exp) 1811
PstI C_TGCA'G (0 Err) - 1 Match(s) found (1.92 sites exp) 1632
XhoI C'TCGA_G (0 Err) - 1 Match(s) found (1.92 sites exp) 1792
XmaI C'CCGG_G (0 Err) - 1 Match(s) found (1.58 sites exp) 1375