F32G8.6 - Blunt Sites in Exons and Introns
BsaXI ACnnnnnCTCC - 1 Cut at 1195
BspGI CTGGAC - 1 Cut at 6
NruI TCG'CGA - 1 Cut at 1311
UbaDI GAACnnnnnnTCC - 1 Cut at 483
BsbI CAACAC - 2 Cuts at 53, 321
DraI TTT'AAA - 2 Cuts at 219, 540
DrdII GAACCA - 2 Cuts at 186, 1169
SspI AAT'ATT - 3 Cuts at 390, 400, 1123
Possible useful sites (RE's cut in Exons):
BspGI (Exon 1 at 6)
BsbI (Exon 1 at 53) (*downstream site in intron)
BsaXI (Exon 4 at 1195)
NruI (Exon 4 at 1311)
Sites within introns (useless):
DrdII (*upstream site in intron), DraI, SspI, UbaDI
1 BspGI BsbI
atg tcc aga att gag aac gaa agc gga ttt ttg tca tct gac gcc gca agt gtt gga tcc
M S R I E N E S G F L S S D A A S V G S
61
gaa gac gat aaa gtt gag atg aag aaa aga aac gga acg att cca aag gaa gat cat ttg
E D D K V E M K K R N G T I P K E D H L
121 164 DrdII
aaa tca atg tgc aat gcc tat cag agc atc att caa cat gtc g|gtgagttcatgtgattctttggt
K S M C N A Y W S I I Q H V
187 DraI
tcggaaagtattagtcgatcaacaacaaatttaaaataagctgtttaagaatagattatagagttctgaaaacgtactgt
267 BsbI
caactgtttacgtcagcagaattaggaaaactttggtttcgataaacatttcaacacactttttgattcattctagagat
347 SspI SspI
tataatgttttagcatttattcattatcagtgactcaaagaatattgataaatattttcccaaatataaatttgaccaag
427 UbaDI
atgttttgaaagtcgatgatctataactgatagttgttattgtctggaaggaagttcagttcaatatattccactctcaa
507 DraI
atcaaaaatcaagatcgaaaattgttttaatttaaagactcatgacaaattgtaatctattacatcacgcaatcaaggta
587
ccctagcacatcatcaggaacttgtgatttcaattgatatatctataacataaatcagaaaaaaataggttgtgtccgtt
667 731
gattataagggaaaaaatgaaaacaaaatacattttcccacgttgactgattttgggaattcag|ga gaa gac atc aat
G E D I N
745
cgt cag gga ctt ctg aaa act cca gaa cgt gct gcc aaa gca atg atg gca ttc aca aag gga
R Q G L L K T P E R A A K A M M A F T K G
808 827 874
tac gat gat caa ctt gat g|gtcagttatagcaatacaacgttaaagctcaaagtttcatttttcag|ag ctc ctc
Y D D Q L D E L L
882
aac gag gca gta ttc gac gag gat cac gat gag atg gtt att gtg aaa gat att gaa atg ttc
N E A V F D E D H D E M V I V K D I E M F
945
tcc ctt tgt gag cat cat tta gtt cca ttt atg gga aaa gtt cat att gga tac att cca aac
S L C E H H L V P F M G K V H I G Y I P N
1008
aag aag gtt ctt ggt ctg tcc aag ttg gca aga ata gtc gag atg ttc agc aga aga ctt caa
K K V L G L S K L A R I V E M F S R R L Q
1071 SspI 1130
g|gtaaattgagaaattacatttttgattggaacctaacctaaacgttcaaatatttcag|tc caa gaa cgt ctt aca
V Q E R L T
1147 DrdII BsaXI
aag caa att gcc acc gca atg gtt caa gct gta caa cca tcc gga gtt gca gtt gtt att gaa
K Q I A T A M V Q A V Q P S G V A V V I E
1210
gct agt cac atg tgt atg gta atg aga gga gtt caa aag atc aat gct tcc acc aca aca tcc
A S H M C M V M R G V Q K I N A S T T T S
1273 NruI
tgc atg ttg gga gta ttc cgt gac gat cca aag act cgc gaa gaa ttc ctt aat ctt atc aac
C M L G V F R D D P K T R E E F L N L I N
1336
aaa cgc taa
K R @