Here you will find a number of protocols and other informative documents we use in the Loer Lab; I will add more as I find time. Many are extremely detailed, and therefore appropriate especially for C. elegans beginnners. Being at a primarily undergraduate, teaching institution, my lab is populated almost exclusively by undergraduate research students. For want of a better descriptor, I have termed these documents "Undergraduate Protocols," to indicate that they take little prior lab or worm experience for granted. As such, some protocols also have Loer lab-specific instructions that will not be useful to others; you may of course modify them for your particular purpose.

• Worm Genetic Nomenclature Basics
• Bacteria for worm food/Seeding plates
• "Chunking" worms - easy strain maintenance
• Quick bleach cleaning of small numbers of worms
• Serotonin Antibody staining
  • short version
  • long version with detailed notes
  • solutions for anti-serotonin protocol
• Formaldehyde induced fluorescence (FIF) for worms, with notes
• Information about dityrosine antibodies

Protocols lacking specific references are derived from well-known worm culturing techniques which tend to vary slightly from lab to lab. Published references containing C. elegans techniques include:

• Brenner S (1974) The genetics of C. elegans. Genetics 77: 71-94.
  
• Epstein HF, Shakes DC (editors) (1995) "Caenorhabditis elegans: modern biological analysis of an organism." Methods in Cell Biology vol. 48. San Diego, California: Academic Press, Inc.
• Sulston J, Hodgkin J (1988) Methods. In: The Nematode Caenorhabditis elegans (W. B. Wood, ed), pp 587-605. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
• Wormbook's Worm Methods section has many methods online, in a citable form.