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MCC - Protocols and Background

The protocols provided here are not recipe books for experiments that students do, they are guides for faculty and students as to the types of experiments that might fit into their CURE containing course and often provide necessary background information on the experimental approaches and types of data analysis that might be used.

  • Traditional Lab Notebook and SOP
  • Electronic Notebook
  • Laboratory Safety
  • Laboratory Math
  • Bacteria Strains in Biochemistry and Molecular Biology
  • Plasmid Elements
  • Agarose Gel Background and Preparation
  • Cloning and Subcloning Stratagies
  • DNA Plasmid Precipitation Protocol
  • Alkaline Lysis Plasmid Prep
  • Bacterial Glycerol Stock Protocol
  • Restriction Enzyme and Agarose Gel Protocol
  • MDH Enzyme Assay Background and Protocol
  • Bacterial Expression Background
  • E. Coli Culture for Protein Expression
  • Bacterial Cell Lysis Protocol
  • Bacterial Transformation (Background and lab version)
  • Z-Comp Transformation
  • Making the most of affinity tags (Publication)
  • Expression Troubleshooting (Industry Handout)
  • Recombinant Protein Expression: Advances and Challenges
  • His Tag (Ni affinity) Purification
  • Qiagen His Tagged Protein Handbook
  • GE Recombinant Protein Purification Handbook
  • Novagen pET System Manual
  • Glutation Affinity (GST) Purification
  • Ni-NTA Bead Regeneration
  • GSH - Agarose Regeneration
  • Bradford Biorad Assay (1.5 ml assay)
  • Bradford Biorad Assay (96 well plate assay)
  • GST Enzyme Protocol
  • Intro Kinetics Module
  • Ligand Binding Module
  • Spectroscopy Protein Folding Module
  • Structural Biology
  • Two Substrate Kinetic Analysis

 

Questions - email: josephprovost@sandiego.edu