Biology 376 Announcements and Handouts

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Biology 376 Announcements and Handouts

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Announcements and Handouts appear here (especially useful if you missed something in class).

Presentations and Reviewing Schedule

Nov. 24
1st talk - Patrick Posadas
2nd talk - Katie Warpinski
3rd talk - Vanessa Ruiz
4th talk - Monica Peterson

1st talk reviewers: Ceara Rathie,
2nd talk reviewers: Derek Kirby, Cassi Thompson
3rd talk reviewers: Ceara Rathie, Cassi Thompson
4th talk reviewers: Derek Kirby,

Dec. 1
1st talk - Ceara Rathie
2nd talk -
3rd talk - Derek Kirby
4th talk - Cassi Thompson

1st talk reviewers: Patrick Posadas, Katie Warpinski
2nd talk reviewers: Vanessa Ruiz, Monica Peterson
3rd talk reviewers: Patrick Posadas, Monica Peterson
4th talk reviewers: Vanessa Ruiz, Katie Warpinski

Echinoderm fertilization lab - experimental plan instructions

You should prepare a plan for a proposed experiment (the subject of your lab report due the following week) that tests the role of an ion such as Na⁺ or Ca⁺⁺ in some aspect of fertilization - the sperm acrosome reaction or egg cortical reaction, etc.

The proposal should be no more than one page in length, and typed. Please bring 2 copies; give one to me at the beginning of lab, or in class earlier in the day (I will try to look at those first, if the lab prep allows).

The proposal should state a specfic hypothesis, such as "Sperm exposed to reduced Na⁺ seawater will show reduced ability to fertilize eggs (perhaps because the acrosome reaction will be triggered prematurely)."

Describe your experimental plan to test the hypothesis - especially your experimental and control conditions (differing concentrations of ions or sperm, exposure of sperm and/or egg before mixing, etc.) and how you will assess the results of the experiments (count percentage of eggs with raised fertilization membranes, percentage of fertilized eggs undergoing normal or abnormal first cleavage, etc.)

If you are repeating experiments described in the text, then indicate this and describe the expected result that would confirm the previous result. If you are testing something new, then indicate what how you might interpret a given result of your experiment.

First Lab will be held Wednesday, September 10

Please obtain the Bio 376 lab manual as soon as possible after the first lecture on Wed, Sept. 3. (Where and when the lab manual will be available will be announced in that class; it will be sold by the department, not the bookstore.) Please come prepared by reading the lab syllabus, the first lab in the manual, and the reserve reading material: "How cells are studied," in Alberts et al., Molecular Biology of the Cell, 3rd Edition, pp 139-148; and viewing the microscopy material on the CD-ROM Vade Mecum (packaged with the textbook). Look at the list of materials you need for lab in the lab manual, and bring these to the first lab. You will conclude the first lab by taking a quiz on microscopy, including material from the assigned reading.

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